TY - JOUR
T1 - A Comparison of Staining Techniques for Somatic Chromosomes of Strawberry
AU - Owen, Henry R.
AU - Miller, A. Raymond
PY - 1993
Y1 - 1993
N2 - A factorial combination of pretreatments, fixatives, and stains was examined to identify the best available method for staining the chromosomes of strawberry (Fragaria × ananassa Duch. ‘Chandler’) root-tip cells. Three pretreatments ( a -bromonaphthalene, p -dichlorobenzene, and 8-hydroxyquinoline), three fixatives (Farmer’s fluid, Carnoy’s fluid, and Newcomer’s fluid), and five stains (acetocarmine, alcoholic hydrochloric-acid carmine, altered carbol fuchsin, lacto-propionic orcein, and leucobasic fuchsin) were tested. Pretreatment with either a -bromonaphthalene (saturated aqueous) or 8-hydroxyquinoline (2 mM) for 5 hours at 14C, overnight fixation in Farmer’s fluid, hydrolysis in 1 N HCl (15 minutes at 60C), and staining with altered carbol fuchsin produced chromosome preparations superior to other treatment combinations. Treatment with a-bromonaphthalene, Farmer’s fluid, and alcoholic hydrochloric-acid carmine (2 days at 25C) also produced acceptable chromosome preparations.
AB - A factorial combination of pretreatments, fixatives, and stains was examined to identify the best available method for staining the chromosomes of strawberry (Fragaria × ananassa Duch. ‘Chandler’) root-tip cells. Three pretreatments ( a -bromonaphthalene, p -dichlorobenzene, and 8-hydroxyquinoline), three fixatives (Farmer’s fluid, Carnoy’s fluid, and Newcomer’s fluid), and five stains (acetocarmine, alcoholic hydrochloric-acid carmine, altered carbol fuchsin, lacto-propionic orcein, and leucobasic fuchsin) were tested. Pretreatment with either a -bromonaphthalene (saturated aqueous) or 8-hydroxyquinoline (2 mM) for 5 hours at 14C, overnight fixation in Farmer’s fluid, hydrolysis in 1 N HCl (15 minutes at 60C), and staining with altered carbol fuchsin produced chromosome preparations superior to other treatment combinations. Treatment with a-bromonaphthalene, Farmer’s fluid, and alcoholic hydrochloric-acid carmine (2 days at 25C) also produced acceptable chromosome preparations.
KW - cytology
KW - Fragaria × ananassa
KW - ploidy level
KW - root-tip squashesA factorial combination of pretreatments
KW - fixatives
KW - and stains was examined to identify the best available method for staining the chromosomes of strawberry (Fragaria × ananassa Duch. ‘Chandler’) root-tip cells. Three pretreatments ( a -bromonaphthalene
KW - p -dichlorobenzene
KW - and 8-hydroxyquinoline)
KW - three fixatives (Farmer’s fluid
KW - Carnoy’s fluid
KW - and Newcomer’s fluid)
KW - and five stains (acetocarmine
KW - alcoholic hydrochloric-acid carmine
KW - altered carbol fuchsin
KW - lacto-propionic orcein
KW - and leucobasic fuchsin) were tested. Pretreatment with either a -bromonaphthalene (saturated aqueous) or 8-hydroxyquinoline (2 mM) for 5 hours at 14C
KW - overnight fixation in Farmer’s fluid
KW - hydrolysis in 1 N HCl (15 minutes at 60C)
KW - and staining with altered carbol fuchsin produced chromosome preparations superior to other treatment combinations. Treatment with a-bromonaphthalene
KW - Farmer’s fluid
KW - and alcoholic hydrochloric-acid carmine (2 days at 25C) also produced acceptable chromosome preparations.
M3 - Article
VL - 28
JO - HortScience
JF - HortScience
ER -