1H NMR Spectroscopy-Based Configurational Analysis of Mono- and Disaccharides and Detection of β-Glucosidase Activity: An Undergraduate Biochemistry Laboratory

Gopal R. Periyannan; Barbara A. Lawrence; Annie E. Egan

doi:10.1021/ED500578C

1H NMR Spectroscopy-Based Configurational Analysis of Mono- and Disaccharides and Detection of β-Glucosidase Activity: An Undergraduate Biochemistry Laboratory

Gopal R. Periyannan, Barbara A. Lawrence, Annie E. Egan

Eastern Illinois University

Producción científica › revisión exhaustiva

Idioma original	American English
Publicación	Journal of Chemical Education
Volumen	92
DOI	https://doi.org/10.1021/ED500578C
Estado	Published - abr 7 2015

Disciplines

Biochemistry
Chemistry
Organic Chemistry

Acceder al documento

10.1021/ED500578CLicencia: Unspecified

Otros archivos y enlaces

Citar esto

@article{b4b1062d1f604fffa5dc8bc2035a0cfd,

title = "1H NMR Spectroscopy-Based Configurational Analysis of Mono- and Disaccharides and Detection of β-Glucosidase Activity: An Undergraduate Biochemistry Laboratory",

author = "Periyannan, \{Gopal R.\} and Lawrence, \{Barbara A.\} and Egan, \{Annie E.\}",

note = "A 1H NMR spectroscopy-based laboratory experiment explores mono- and disaccharide structural chemistry, and the enzyme-substrate specificity of glycosidic bond cleavage by β-glucosidase towards cellobiose (β-linked gluco-disaccharide) and maltose (α-linked gluco-disaccharide). Structural differences between cellobiose, maltose, and the glycolytic product glucose are established in terms of chemical shift changes and J-coupling constants of the anomeric proton peaks.",

year = "2015",

month = apr,

day = "7",

doi = "10.1021/ED500578C",

language = "American English",

volume = "92",

journal = "Journal of Chemical Education",

}

TY - JOUR

T1 - 1H NMR Spectroscopy-Based Configurational Analysis of Mono- and Disaccharides and Detection of β-Glucosidase Activity: An Undergraduate Biochemistry Laboratory

AU - Periyannan, Gopal R.

AU - Lawrence, Barbara A.

AU - Egan, Annie E.

N1 - A 1H NMR spectroscopy-based laboratory experiment explores mono- and disaccharide structural chemistry, and the enzyme-substrate specificity of glycosidic bond cleavage by β-glucosidase towards cellobiose (β-linked gluco-disaccharide) and maltose (α-linked gluco-disaccharide). Structural differences between cellobiose, maltose, and the glycolytic product glucose are established in terms of chemical shift changes and J-coupling constants of the anomeric proton peaks.

PY - 2015/4/7

Y1 - 2015/4/7

UR - http://ui.adsabs.harvard.edu/abs/2015JChEd..92.1244P/abstract

UR - https://pubs.acs.org/doi/abs/10.1021/ed500578c

U2 - 10.1021/ED500578C

DO - 10.1021/ED500578C

M3 - Article

VL - 92

JO - Journal of Chemical Education

JF - Journal of Chemical Education

ER -