Carbon Monoxide-Dependent Chemolithotrophic Growth of Clostridium thermoautotrophicum

M. Dean Savage; Zhongren Wu; Steven L. Daniel; Leon L. Lundie; Harold L. Drake

Carbon Monoxide-Dependent Chemolithotrophic Growth of Clostridium thermoautotrophicum

M. Dean Savage, Zhongren Wu, Steven L. Daniel, Leon L. Lundie, Harold L. Drake

Eastern Illinois University

Research output: Contribution to journal › Article › peer-review

Abstract

The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO --> CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; Y H2 , was considerably lower than Y CO Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.

Original language	American English
Journal	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume	53
State	Published - Aug 1987

Disciplines

Bacteriology
Environmental Microbiology and Microbial Ecology
Microbial Physiology
Microbiology

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Cite this

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title = "Carbon Monoxide-Dependent Chemolithotrophic Growth of Clostridium thermoautotrophicum",

abstract = " The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO --> CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; Y H2 , was considerably lower than Y CO Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.",

author = "Savage, {M. Dean} and Zhongren Wu and Daniel, {Steven L.} and Lundie, {Leon L.} and Drake, {Harold L.}",

year = "1987",

month = aug,

language = "American English",

volume = "53",

journal = "APPLIED AND ENVIRONMENTAL MICROBIOLOGY",

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TY - JOUR

T1 - Carbon Monoxide-Dependent Chemolithotrophic Growth of Clostridium thermoautotrophicum

AU - Savage, M. Dean

AU - Wu, Zhongren

AU - Daniel, Steven L.

AU - Lundie, Leon L.

AU - Drake, Harold L.

PY - 1987/8

Y1 - 1987/8

N2 - The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO --> CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; Y H2 , was considerably lower than Y CO Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.

AB - The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO --> CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; Y H2 , was considerably lower than Y CO Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.

M3 - Article

VL - 53

JO - APPLIED AND ENVIRONMENTAL MICROBIOLOGY

JF - APPLIED AND ENVIRONMENTAL MICROBIOLOGY

ER -