1H NMR Spectroscopy-Based Configurational Analysis of Mono- and Disaccharides and Detection of β-Glucosidase Activity: An Undergraduate Biochemistry Laboratory

TY - JOUR

T1 - 1H NMR Spectroscopy-Based Configurational Analysis of Mono- and Disaccharides and Detection of β-Glucosidase Activity: An Undergraduate Biochemistry Laboratory

AU - Periyannan, Gopal R.

AU - Lawrence, Barbara A.

AU - Egan, Annie E.

N1 - A 1H NMR spectroscopy-based laboratory experiment explores mono- and disaccharide structural chemistry, and the enzyme-substrate specificity of glycosidic bond cleavage by β-glucosidase towards cellobiose (β-linked gluco-disaccharide) and maltose (α-linked gluco-disaccharide). Structural differences between cellobiose, maltose, and the glycolytic product glucose are established in terms of chemical shift changes and J-coupling constants of the anomeric proton peaks.

PY - 2015/4/7

Y1 - 2015/4/7

UR - http://ui.adsabs.harvard.edu/abs/2015JChEd..92.1244P/abstract

UR - https://pubs.acs.org/doi/abs/10.1021/ed500578c

U2 - 10.1021/ED500578C

DO - 10.1021/ED500578C

M3 - Article

VL - 92

JO - Journal of Chemical Education

JF - Journal of Chemical Education

ER -